Sperm Preservation by Freeze-Drying for the Conservation of Wild Animals
DOI: 10.1371/journal.pone.0113381Research by
Takehito Kaneko, Hideyuki Ito, Hidefusa Sakamoto, Manabu Onuma, Miho Inoue‐Murayama
Reports pronuclear formation in mouse oocytes after injection of freeze-dried sperm from one giraffe, distinguishing this endpoint from giraffe reproductive success.
Publication details
Study facts
- Method
- Laboratory freeze-drying study with heterologous mouse-oocyte assay
- Location
- Japanese zoo-derived and opportunistic specimens; Kyoto laboratory
- Species
- Reticulated giraffe among five sperm-donor species; mouse recipient oocytes
- Sample
- One giraffe donor; 24 injected mouse oocytes, 12 surviving
- Period
- One-month sperm storage; calendar collection dates not reported
Full citation
Takehito Kaneko, Hideyuki Ito, Hidefusa Sakamoto, Manabu Onuma, Miho Inoue‐Murayama (2014). Sperm Preservation by Freeze-Drying for the Conservation of Wild Animals. PLoS ONE. https://doi.org/10.1371/journal.pone.0113381
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