Sperm Preservation by Freeze-Drying for the Conservation of Wild Animals

DOI: 10.1371/journal.pone.0113381

Research by

Takehito Kaneko, Hideyuki Ito, Hidefusa Sakamoto, Manabu Onuma, Miho Inoue‐Murayama

Reports pronuclear formation in mouse oocytes after injection of freeze-dried sperm from one giraffe, distinguishing this endpoint from giraffe reproductive success.

Publication details

Study facts

Method
Laboratory freeze-drying study with heterologous mouse-oocyte assay
Location
Japanese zoo-derived and opportunistic specimens; Kyoto laboratory
Species
Reticulated giraffe among five sperm-donor species; mouse recipient oocytes
Sample
One giraffe donor; 24 injected mouse oocytes, 12 surviving
Period
One-month sperm storage; calendar collection dates not reported

Full citation

Takehito Kaneko, Hideyuki Ito, Hidefusa Sakamoto, Manabu Onuma, Miho Inoue‐Murayama (2014). Sperm Preservation by Freeze-Drying for the Conservation of Wild Animals. PLoS ONE. https://doi.org/10.1371/journal.pone.0113381

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